Two differentially regulated class II chitinases from parsley.
Identifieur interne : 002772 ( Main/Exploration ); précédent : 002771; suivant : 002773Two differentially regulated class II chitinases from parsley.
Auteurs : Y. Ponath [Allemagne] ; H. Vollberg ; K. Hahlbrock ; E. KombrinkSource :
- Biological chemistry [ 1431-6730 ] ; 2000.
Descripteurs français
- KwdFr :
- ADN complémentaire (composition chimique), ADN complémentaire (génétique), ADN complémentaire (isolement et purification), ARN messager (métabolisme), Alignement de séquences (MeSH), Apiaceae (croissance et développement), Apiaceae (enzymologie), Apiaceae (immunologie), Chitinase (génétique), Chitinase (isolement et purification), Chitinase (pharmacologie), Distribution tissulaire (MeSH), Données de séquences moléculaires (MeSH), Gènes de plante (génétique), Hybridation in situ (MeSH), Immunité innée (MeSH), Immunohistochimie (MeSH), Isoenzymes (MeSH), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Phylogenèse (MeSH), Protéines végétales (génétique), Protéines végétales (isolement et purification), Protéines végétales (pharmacologie), Régulation de l'expression des gènes végétaux (MeSH), Séquence nucléotidique (MeSH).
- MESH :
- composition chimique : ADN complémentaire.
- croissance et développement : Apiaceae.
- enzymologie : Apiaceae.
- génétique : ADN complémentaire, Chitinase, Gènes de plante, Maladies des plantes, Protéines végétales.
- immunologie : Apiaceae.
- isolement et purification : ADN complémentaire, Chitinase, Protéines végétales.
- microbiologie : Maladies des plantes.
- métabolisme : ARN messager.
- pharmacologie : Chitinase, Protéines végétales.
- Alignement de séquences, Distribution tissulaire, Données de séquences moléculaires, Hybridation in situ, Immunité innée, Immunohistochimie, Isoenzymes, Phylogenèse, Régulation de l'expression des gènes végétaux, Séquence nucléotidique.
English descriptors
- KwdEn :
- Apiaceae (enzymology), Apiaceae (growth & development), Apiaceae (immunology), Base Sequence (MeSH), Chitinases (genetics), Chitinases (isolation & purification), Chitinases (pharmacology), DNA, Complementary (chemistry), DNA, Complementary (genetics), DNA, Complementary (isolation & purification), Gene Expression Regulation, Plant (MeSH), Genes, Plant (genetics), Immunity, Innate (MeSH), Immunohistochemistry (MeSH), In Situ Hybridization (MeSH), Isoenzymes (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Proteins (genetics), Plant Proteins (isolation & purification), Plant Proteins (pharmacology), RNA, Messenger (metabolism), Sequence Alignment (MeSH), Tissue Distribution (MeSH).
- MESH :
- chemical , chemistry : DNA, Complementary.
- chemical , genetics : Chitinases, DNA, Complementary, Plant Proteins.
- enzymology : Apiaceae.
- genetics : Genes, Plant, Plant Diseases.
- growth & development : Apiaceae.
- immunology : Apiaceae.
- chemical , isolation & purification : Chitinases, DNA, Complementary, Plant Proteins.
- chemical , metabolism : RNA, Messenger.
- microbiology : Plant Diseases.
- chemical , pharmacology : Chitinases, Plant Proteins.
- Base Sequence, Gene Expression Regulation, Plant, Immunity, Innate, Immunohistochemistry, In Situ Hybridization, Isoenzymes, Molecular Sequence Data, Phylogeny, Sequence Alignment, Tissue Distribution.
Abstract
Two distinct cDNA clones, PcCHI1 and PcCHI2, with high sequence similarity to plant chitinases were isolated from parsley (Petroselinum crispum), expressed in Escherichia coli, and the encoded proteins functionally identified as endochitinases. Different expression patterns of the corresponding mRNAs and proteins in infected and uninfected parsley plants indicated distinct roles of the two isoforms in both pathogen defense and plant development. Infection of parsley leaf buds with Phytophthora sojae resulted in the rapid, transient and highly localized accumulation of PcCHI1 mRNA and protein around infection sites, whereas PcCHI2 mRNA and protein were systemically induced at later infection stages. Similar differences in the timing of induction were observed in elicitor-treated, suspension-cultured parsley cells. In uninfected plants, PcCHI1 mRNA was particularly abundant in the transmitting tract of healthy flowers, suggesting a role in the constitutive protection of susceptible transmitting tissue of the style against pathogen ingress and/or in the fertilization process, possibly by affecting pollen tube growth. Localization of PcCHI2 mRNA and protein in the parenchymatic collenchyme of young pedicels may indicate a function in the constitutive protection of this tissue. In addition to such distinct roles of PcCHI1 and PcCHI2 in preformed and induced pathogen defense, both chitinases may have endogenous regulatory functions in plant development.
DOI: 10.1515/BC.2000.087
PubMed: 11030424
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Two differentially regulated class II chitinases from parsley.</title><author><name sortKey="Ponath, Y" sort="Ponath, Y" uniqKey="Ponath Y" first="Y" last="Ponath">Y. Ponath</name><affiliation wicri:level="3"><nlm:affiliation>Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Vollberg, H" sort="Vollberg, H" uniqKey="Vollberg H" first="H" last="Vollberg">H. Vollberg</name></author><author><name sortKey="Hahlbrock, K" sort="Hahlbrock, K" uniqKey="Hahlbrock K" first="K" last="Hahlbrock">K. Hahlbrock</name></author><author><name sortKey="Kombrink, E" sort="Kombrink, E" uniqKey="Kombrink E" first="E" last="Kombrink">E. Kombrink</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:11030424</idno><idno type="pmid">11030424</idno><idno type="doi">10.1515/BC.2000.087</idno><idno type="wicri:Area/Main/Corpus">002830</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002830</idno><idno type="wicri:Area/Main/Curation">002830</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002830</idno><idno type="wicri:Area/Main/Exploration">002830</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Two differentially regulated class II chitinases from parsley.</title><author><name sortKey="Ponath, Y" sort="Ponath, Y" uniqKey="Ponath Y" first="Y" last="Ponath">Y. Ponath</name><affiliation wicri:level="3"><nlm:affiliation>Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Vollberg, H" sort="Vollberg, H" uniqKey="Vollberg H" first="H" last="Vollberg">H. Vollberg</name></author><author><name sortKey="Hahlbrock, K" sort="Hahlbrock, K" uniqKey="Hahlbrock K" first="K" last="Hahlbrock">K. Hahlbrock</name></author><author><name sortKey="Kombrink, E" sort="Kombrink, E" uniqKey="Kombrink E" first="E" last="Kombrink">E. Kombrink</name></author></analytic><series><title level="j">Biological chemistry</title><idno type="ISSN">1431-6730</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apiaceae (enzymology)</term><term>Apiaceae (growth & development)</term><term>Apiaceae (immunology)</term><term>Base Sequence (MeSH)</term><term>Chitinases (genetics)</term><term>Chitinases (isolation & purification)</term><term>Chitinases (pharmacology)</term><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (genetics)</term><term>DNA, Complementary (isolation & purification)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (genetics)</term><term>Immunity, Innate (MeSH)</term><term>Immunohistochemistry (MeSH)</term><term>In Situ Hybridization (MeSH)</term><term>Isoenzymes (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (isolation & purification)</term><term>Plant Proteins (pharmacology)</term><term>RNA, Messenger (metabolism)</term><term>Sequence Alignment (MeSH)</term><term>Tissue Distribution (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (composition chimique)</term><term>ADN complémentaire (génétique)</term><term>ADN complémentaire (isolement et purification)</term><term>ARN messager (métabolisme)</term><term>Alignement de séquences (MeSH)</term><term>Apiaceae (croissance et développement)</term><term>Apiaceae (enzymologie)</term><term>Apiaceae (immunologie)</term><term>Chitinase (génétique)</term><term>Chitinase (isolement et purification)</term><term>Chitinase (pharmacologie)</term><term>Distribution tissulaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Gènes de plante (génétique)</term><term>Hybridation in situ (MeSH)</term><term>Immunité innée (MeSH)</term><term>Immunohistochimie (MeSH)</term><term>Isoenzymes (MeSH)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Phylogenèse (MeSH)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (isolement et purification)</term><term>Protéines végétales (pharmacologie)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chitinases</term><term>DNA, Complementary</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN complémentaire</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Apiaceae</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Apiaceae</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Apiaceae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Plant</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Apiaceae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Chitinase</term><term>Gènes de plante</term><term>Maladies des plantes</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Apiaceae</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Apiaceae</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Chitinases</term><term>DNA, Complementary</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN complémentaire</term><term>Chitinase</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chitinase</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chitinases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Gene Expression Regulation, Plant</term><term>Immunity, Innate</term><term>Immunohistochemistry</term><term>In Situ Hybridization</term><term>Isoenzymes</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Alignment</term><term>Tissue Distribution</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Distribution tissulaire</term><term>Données de séquences moléculaires</term><term>Hybridation in situ</term><term>Immunité innée</term><term>Immunohistochimie</term><term>Isoenzymes</term><term>Phylogenèse</term><term>Régulation de l'expression des gènes végétaux</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two distinct cDNA clones, PcCHI1 and PcCHI2, with high sequence similarity to plant chitinases were isolated from parsley (Petroselinum crispum), expressed in Escherichia coli, and the encoded proteins functionally identified as endochitinases. Different expression patterns of the corresponding mRNAs and proteins in infected and uninfected parsley plants indicated distinct roles of the two isoforms in both pathogen defense and plant development. Infection of parsley leaf buds with Phytophthora sojae resulted in the rapid, transient and highly localized accumulation of PcCHI1 mRNA and protein around infection sites, whereas PcCHI2 mRNA and protein were systemically induced at later infection stages. Similar differences in the timing of induction were observed in elicitor-treated, suspension-cultured parsley cells. In uninfected plants, PcCHI1 mRNA was particularly abundant in the transmitting tract of healthy flowers, suggesting a role in the constitutive protection of susceptible transmitting tissue of the style against pathogen ingress and/or in the fertilization process, possibly by affecting pollen tube growth. Localization of PcCHI2 mRNA and protein in the parenchymatic collenchyme of young pedicels may indicate a function in the constitutive protection of this tissue. In addition to such distinct roles of PcCHI1 and PcCHI2 in preformed and induced pathogen defense, both chitinases may have endogenous regulatory functions in plant development.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11030424</PMID><DateCompleted><Year>2001</Year><Month>03</Month><Day>22</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1431-6730</ISSN><JournalIssue CitedMedium="Print"><Volume>381</Volume><Issue>8</Issue><PubDate><Year>2000</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Biological chemistry</Title><ISOAbbreviation>Biol Chem</ISOAbbreviation></Journal><ArticleTitle>Two differentially regulated class II chitinases from parsley.</ArticleTitle><Pagination><MedlinePgn>667-78</MedlinePgn></Pagination><Abstract><AbstractText>Two distinct cDNA clones, PcCHI1 and PcCHI2, with high sequence similarity to plant chitinases were isolated from parsley (Petroselinum crispum), expressed in Escherichia coli, and the encoded proteins functionally identified as endochitinases. Different expression patterns of the corresponding mRNAs and proteins in infected and uninfected parsley plants indicated distinct roles of the two isoforms in both pathogen defense and plant development. Infection of parsley leaf buds with Phytophthora sojae resulted in the rapid, transient and highly localized accumulation of PcCHI1 mRNA and protein around infection sites, whereas PcCHI2 mRNA and protein were systemically induced at later infection stages. Similar differences in the timing of induction were observed in elicitor-treated, suspension-cultured parsley cells. In uninfected plants, PcCHI1 mRNA was particularly abundant in the transmitting tract of healthy flowers, suggesting a role in the constitutive protection of susceptible transmitting tissue of the style against pathogen ingress and/or in the fertilization process, possibly by affecting pollen tube growth. Localization of PcCHI2 mRNA and protein in the parenchymatic collenchyme of young pedicels may indicate a function in the constitutive protection of this tissue. In addition to such distinct roles of PcCHI1 and PcCHI2 in preformed and induced pathogen defense, both chitinases may have endogenous regulatory functions in plant development.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ponath</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vollberg</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Hahlbrock</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Kombrink</LastName><ForeName>E</ForeName><Initials>E</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Biol Chem</MedlineTA><NlmUniqueID>9700112</NlmUniqueID><ISSNLinking>1431-6730</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007527">Isoenzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="C421910">CHI1-1 protein, Petroselinum crispum</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="C421911">CHI1-2 protein, Petroselinum crispum</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="D002688">Chitinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019661" MajorTopicYN="N">Apiaceae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002688" MajorTopicYN="N">Chitinases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017403" MajorTopicYN="N">In Situ Hybridization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007527" MajorTopicYN="N">Isoenzymes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014018" MajorTopicYN="N">Tissue Distribution</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>10</Month><Day>13</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>3</Month><Day>27</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>10</Month><Day>13</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11030424</ArticleId><ArticleId IdType="doi">10.1515/BC.2000.087</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>District de Cologne</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Cologne</li></settlement></list><tree><noCountry><name sortKey="Hahlbrock, K" sort="Hahlbrock, K" uniqKey="Hahlbrock K" first="K" last="Hahlbrock">K. Hahlbrock</name><name sortKey="Kombrink, E" sort="Kombrink, E" uniqKey="Kombrink E" first="E" last="Kombrink">E. Kombrink</name><name sortKey="Vollberg, H" sort="Vollberg, H" uniqKey="Vollberg H" first="H" last="Vollberg">H. Vollberg</name></noCountry><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Ponath, Y" sort="Ponath, Y" uniqKey="Ponath Y" first="Y" last="Ponath">Y. Ponath</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002772 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002772 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:11030424
|texte= Two differentially regulated class II chitinases from parsley.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:11030424" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |